Hotstart Master Mix with dye, 1.25ml/kit

The HotStar Taq Mastermix which include the HotStar DNA Polymerase,PCR Buffer, Mg2+ , dNTPs, the blue dye and the stabilizing agent. The HotStarTaq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is activated in 10 minute incubation at 95ﾰC. The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5?to 3?DNA polymerase activity and 5?to 3?exonuclease activity without 3?-5?exonuclease activity. The extending speed is 1 kb/30 sec. There is an "A" on 3?end.The PCR product can be cloned in TA vector.