Fast EvaGreen Master Mix for qPCR (5000 rxn, 50x1 ml)

Fast Evagreen® qPCR Master Mix is a PCR reagent suitable for qPCR using a fast cycling protocol. The reagent system uses two of our breakthrough PCR technologies, EvaGreen® qPCR dye and Cheetah^TMTaq hotstart DNA polymerase, to deliver superior results when compared to other commercial master mixes.

• Evagreen® dye: EvaGreen® DNA-binding dye selectively binds to dsDNA via a novel "release-on-demand" mechanism that ensures low PCR inhibition and thus permits the dye to be used at a relatively high concentration for melt curve analysis signal. Because the dye is spectrally similar to FAM or SYBR® Green I, the dye is compatible with all commercial qPCR instruments. Moreover, unlike SYBR® Green I and SYBR GreenER®, Evagreen® dye is extremely stable. Finally, Evagreen® dye is nonmutagenic and noncytotoxic.

• Cheetah^TM Taq: Cheetah^TM Taq is a new, chemically modified hotstart DNA polymerase. Unlike Amplitaq Gold®, Cheetah^TM Taq can be activated in as little as two minutes under standard hotstart condition (i.e., 94°C in pH 8-9 Tris) with high activity recovery. Also unlike antibody-based hotstart polymerases, such as Platinum® Taq, Cheetah^TM Taq is completely inactive at room temperature and has intrinsically low DNA contamination. In addition, Cheetah^TM Taq is generally more stable at -20°C or 4°C than AmpliTaq Gold®, ensuring that the master mix maintain its peak performance following storage at low temperature.

Fast EvaGreen® Qpcr Master Mix is also available in 500-reaction size (cat#31003-1) and 5,000-reaction size (cat#31003-2).