Biotin-Protein L
| Product Name | Biotin-Protein L | |
|---|---|---|
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Description
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Protein L is a cell surface protein from Peptostreptoccocus magnusthat binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. | |
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Concentration
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1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. | |
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Reconstitution
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Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml. | |
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Storage
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The product is stable in lyophilized form if stored at -20°C or below. The reconstituted product can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. | |
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Application
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Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:100,000 Other: user-optimized |
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Document-DATASHEET: 2900_20070208033901.PDF (PDF)
- SKU: M00097
- Size: 1 mg
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$107.80