His Mini Purification and Detection Kit

  • His Mini Purification and Detection Kit

His Mini Purification and Detection Kit



Description:

GenScript His-Tag Mini Purification and Detection Kit was developed for the efficient small scale purification or immunoprecipitation (IP) and detection of His-tagged proteins in eukaryotic and prokaryotic expression systems. Superparamagnetic beads pre-charged with nickel are ready for quick binding of His-tagged proteins. The Magnetic stand in the kit facilitates Magbeads isolation. After purification/IP, His tagged protein could be detected by THETM His tag monoclonal antibody (mAb) included in the system. This kit also offers two His tagged proteins (26.1kDa and 51.6kDa, respectively) as positive control and general molecular weight (M.W) ladder for Western blot detection. 

Key Features:
  • Complete system: This kit offers all key components needed for His-tagged protein purification/IP and detection, which provides One-stop Convenience. The high binding capacity and user friendly protocol makes your protein isolation and detection simple and cost-effective.
  • High Capacity: Bind 5-20 mg of 6xHis tagged protein per milliliter (ml) settled Ni-charged MagBeads.
  • High Versatility: Purify proteins under native or denatured conditions, N- or C- terminal His-tagged with various length (Hexa-His, Penta-His and Tetra-His) from eukaryotic and prokaryotic expression system.
  • High Compatibility: Tolerable with various reagents involved in the purification process.
  • High Sensitivity: The ultra TheTMHis Tag mAb ensures detection of as low as 1 ng His tagged protein in Western Blot assay.


Applications:
  • Purification and detection of His-tagged proteins under native conditions.
  • Purification and detection of His-tagged proteins under denaturing conditions.
  • Screen His-fusion protein expression for expression optimization.


Storage:

  • Please store the components at recommended conditions after receiving the kit.
  • The kit components are stable for at least one year unless specified, when stored under recommended conditions.
  • Use only before expiration date.

 

 

Technical Manual


 

Components
Ni-MagBeads 8 ml (25% slurry)
THETM His mAb 20 μg
Goat anti-mouse IgG (H 100 μg
His tag Protein Positive Control 20 μg
Magnetic Separation Rack 1 piece

 

 

Document

 

  • SKU: L00438
  • Size: kit
  • $493.90